Article
Determination of unique amino acid substitutions in protein variants by peptide mass mapping with FT-ICR MS.
Journal of the American Society for Mass Spectrometry - 1 Apr 2006
Tanaka Katsuhiro, Takenaka Shigeo, Tsuyama Shingo, Wada Yoshinao
Abstract excerpt
Peptide mass mapping plays a central role in the structural characterization of protein variants with single amino acid substitutions. Among the 20 standard amino acids found in living organisms, 18, all but Leu and Ile, differ from each other in molecular mass. The mass differences between amino acids range from 0.0364 to 129.0578 Da. The mass of the mutated peptide or the difference between normal and mutated...
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