Article
Crystal structure of the cyan fluorescent protein Cerulean-S175G.
Acta crystallographica. Section F, Structural biology communications - 1 Jul 2016
Park Sang Wook, Kang Sunghyun, Yoon Tae Sung
Abstract excerpt
Enhanced cyan fluorescent protein (ECFP) was derived from Aequorea victoria green fluorescent protein (avGFP), notably with S65T/Y66W mutations. Its chromophore consists of a tripeptide comprised of Thr65, Trp66 and Gly67 (TWG) residues, while that of avGFP consists of a Ser65, Tyr66 and Gly67 (SYG) tripeptide. Cerulean and SCFP3A were derived from ECFP-S72A/H148D (a double mutation) with additional Y145A and...
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