Article
Cerulean, Venus, and VenusY67C FRET reference standards.
Biophysical journal - 15 Dec 2006
Koushik Srinagesh V, Chen Huanmian, Thaler Christopher, Puhl Henry L, Vogel Steven S
Abstract excerpt
Förster's resonance energy transfer (FRET) can be used to study protein-protein interactions in living cells. Numerous methods to measure FRET have been devised and implemented; however, the accuracy of these methods is unknown, which makes interpretation of FRET efficiency values difficult if not impossible. This problem exists due to the lack of standards with known FRET efficiencies that can be used to...
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