Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime.

doi:10.1016/j.bpj.2015.06.018

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Article

Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime.

Biophysical journal - 21 Jul 2015

Sarkisyan Karen S, Goryashchenko Alexander S, Lidsky Peter V, Gorbachev Dmitry A, Bozhanova Nina G, Gorokhovatsky Andrey Yu, Pereverzeva Alina R, Ryumina Alina P, Zherdeva Victoria V, Savitsky Alexander P, Solntsev Kyril M, Bommarius Andreas S, Sharonov George V, Lindquist Jake R, Drobizhev Mikhail, Hughes Thomas E, Rebane Aleksander, Lukyanov Konstantin A, Mishin Alexander S

Abstract excerpt

Spectral diversity of fluorescent proteins, crucial for multiparameter imaging, is based mainly on chemical diversity of their chromophores. Recently we have reported, to our knowledge, a new green fluorescent protein WasCFP-the first fluorescent protein with a tryptophan-based chromophore in the anionic state. However, only a small portion of WasCFP molecules exists in the anionic state at physiological...

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Identifiers

PMID: 26200874
DOI: 10.1016/j.bpj.2015.06.018
PMCID: PMC4621817

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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