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Article

Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects.

BMC biotechnology - 8 Nov 2019

Zheng Guoxing, Zhu Qingqing, Dong Junchao, Lin Xin, Zhu Chengming

Abstract excerpt

BACKGROUND: Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated tool for genetic modifications. However, utilization of CRISPR/Cas9 to introduce point mutations and the exclusion of off-target effects in mice remain challenging. TP53-R175 is one of the most...

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PMID: 31703569
DOI: 10.1186/s12896-019-0573-z
PMCID: PMC6839086

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