Article
Pichia pastoris Mut(S) strains are prone to misincorporation of O-methyl-L-homoserine at methionine residues when methanol is used as the sole carbon source.
Microbial cell factories - 7 Jun 2016
Schotte Peter, Dewerte Isabelle, De Groeve Manu, De Keyser Saskia, De Brabandere Veronique, Stanssens Patrick
Abstract excerpt
BACKGROUND: Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. Several P. pastoris engineered strains and mutants have been developed to improve the performance of the expression system. Yield and quality of a recombinant product are important parameters to monitor during the host selection and...
Topics
- Aldehyde Oxidase
- Carbon
- Chromatography, High Pressure Liquid
- Fungal Proteins
- Genotype
- Homoserine
- Methanol
- Methionine
- Pichia
- Plasmids
- Single-Domain Antibodies