Article
Evaluation of Mut+ and MutS Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration.
Biotechnology progress - 1 Jan 2000
Pla Itzcoatl A, Damasceno Leonardo M, Vannelli Todd, Ritter Gerd, Batt Carl A, Shuler Michael L
Abstract excerpt
Extracellular secretion of over 4 g x L(-1) of the A33 scFv antibody fragment was achieved in Pichia pastoris at the 10 L bioreactor scale using minimal medium and feedback control of the methanol concentration. Since methanol acts as both inducer and carbon source, its close regulation is a crucial factor in achieving optimal fermentation conditions. The antibody fragment production levels of both Mut+ and MutS...
Topics
- Antibodies, Monoclonal
- Bioreactors
- Feedback, Physiological
- Fermentation
- Immunoglobulin Variable Region
- Methanol
- Models, Biological
- Phenotype
- Pichia
- Time Factors