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Article

Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.

Journal of biotechnology - 6 Apr 2005

Cos Oriol, Serrano Alicia, Montesinos José Luis, Ferrer Pau, Cregg James M, Valero Francisco

Abstract excerpt

An important number of heterologous proteins have been produced in the methylotrophic yeast Pichia pastoris using the alcohol oxidase promoter. Two factors that drastically influence protein production and cultivation process development in this system are gene dosage and methanol assimilation capacity of the host strain (Mut phenotype). Using a battery of four strains which secrete a Rhizopus oryzae lipase...

Topics

Cell Proliferation
Enzyme Activation
Gene Dosage
Genetic Enhancement
Lipase
Methanol
Phenotype
Pichia
Protein Engineering
Recombinant Proteins
Rhizopus

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Identifiers

PMID: 15748759
DOI: 10.1016/j.jbiotec.2004.12.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15748759-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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