Construction and characterization of Pichia pastoris strains for labeling aromatic amino acids in recombinant proteins.

doi:10.1016/j.pep.2004.11.008

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Article

Construction and characterization of Pichia pastoris strains for labeling aromatic amino acids in recombinant proteins.

Protein expression and purification - 1 Jun 2005

Whittaker Mei M, Whittaker James W

Abstract excerpt

Strains of the methylotrophic yeast Pichia pastoris auxotrophic for the aromatic amino acids (tyrosine, phenylalanine, and tryptophan) have been constructed by targeted gene disruption for protein labeling applications. Three strains, with defects in ARO1 (coding for a homolog of the arom pentafu...

Topics

Amino Acids, Aromatic
Genotype
Pichia
Recombinant Proteins
Staining and Labeling

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