Article
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Microbial cell factories - 17 Mar 2017
Moser Josef W, Prielhofer Roland, Gerner Samuel M, Graf Alexandra B, Wilson Iain B H, Mattanovich Diethard, Dragosits Martin
Abstract excerpt
BACKGROUND: Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order to improve strains for biotechnological purposes. In this context, the impact of long-term carbon so...
Topics
- Alcohol Oxidoreductases
- Batch Cell Culture Techniques
- Biotechnology
- Cloning, Molecular
- Culture Media
- Directed Molecular Evolution
- Methanol
- Mutation
- Pichia
- Pilot Projects
- Promoter Regions, Genetic
- Recombinant Proteins