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Article

Tethered bilayer membranes as a complementary tool for functional and structural studies: The pyolysin case.

Biochimica et biophysica acta - 1 Sept 2016

Preta Giulio, Jankunec Marija, Heinrich Frank, Griffin Sholeem, Sheldon Iain Martin, Valincius Gintaras

Abstract excerpt

We demonstrate the use of tethered bilayer lipid membranes (tBLMs) as an experimental platform for functional and structural studies of membrane associated proteins by electrochemical techniques. The reconstitution of the cholesterol-dependent cytolysin (CDC) pyolysin (PLO) from Trueperella pyogenes into tBLMs was followed in real-time by electrochemical impedance spectroscopy (EIS). Changes of the EIS parameters...

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Identifiers

PMID: 27211243
DOI: 10.1016/j.bbamem.2016.05.016

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AFM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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