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Article

Reconstitution of cholesterol-dependent vaginolysin into tethered phospholipid bilayers: implications for bioanalysis.

PloS one - 1 Jan 2013

Budvytyte Rima, Pleckaityte Milda, Zvirbliene Aurelija, Vanderah David J, Valincius Gintaras

Abstract excerpt

Functional reconstitution of the cholesterol-dependent cytolysin vaginolysin (VLY) from Gardnerella vaginalis into artificial tethered bilayer membranes (tBLMs) has been accomplished. The reconstitution of VLY was followed in real-time by electrochemical impedance spectroscopy (EIS). Changes of the EIS parameters of the tBLMs upon exposure to VLY solutions were consistent with the formation of water-filled pores...

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Identifiers

PMID: 24349307
DOI: 10.1371/journal.pone.0082536
PMCID: PMC3862629

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