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Isothermal Detection of Single-Nucleotide Variants via pH-Mediated Toehold-Controlled Ligation on Double-Stranded DNA.

ACS synthetic biology - 15 May 2026

Gong Yu, Chen Yun, Shu Xiangrong, Wang Yingchen, Zhou Xuanye, Shi Yangjing, Qi Hao

Abstract excerpt

Single-nucleotide variants (SNVs) are essential biomarkers in molecular diagnostics, yet their rapid and precise detection remains difficult due to the dependence on thermally driven DNA denaturation and instrument-intensive workflows. Here, we introduce a simple and cost-effective physicochemical strategy that exploits the strong pH sensitivity of DNA duplex stability to enable direct SNV detection on...

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PMID: 42044899
DOI: 10.1021/acssynbio.6c00046

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