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Article

Overexpression of the pullulan synthetase gene enhanced pullulan production and its molecular weight by a mutant of Aureobasidium melanogenum P16.

International journal of biological macromolecules - 1 Dec 2024

Hansali Khalef, Wang Peng, Zhao Shou-Feng, Wang Peng, Ma Zai-Chao, Chi Zhe, Chi Zhen-Ming

Abstract excerpt

The pullulan synthetase gene (PUL1), involved in pullulan biosynthesis in Aureobasidium species, remains poorly understood. The open reading frame (ORF) of the PUL1 gene from the high pullulan-producing yeast Aureobasidium melanogenum P16 strain was cloned and characterized. The ORF of the PUL1 gene was determined to be 592 bp in length, encoding 178 amino acid residues. It was observed that an intron of 55 bp...

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PMID: 39486724
DOI: 10.1016/j.ijbiomac.2024.137013

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