Article
SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture.
Nature cell biology - 1 Apr 2016
Hilbert Manuel, Noga Akira, Frey Daniel, Hamel Virginie, Guichard Paul, Kraatz Sebastian H W, Pfreundschuh Moritz, Hosner Sarah, Flückiger Isabelle, Jaussi Rolf, Wieser Mara M, Thieltges Katherine M, Deupi Xavier, Müller Daniel J, Kammerer Richard A, Gönczy Pierre, Hirono Masafumi, Steinmetz Michel O
Abstract excerpt
Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro...
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