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Article

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy.

Journal of visualized experiments : JoVE - 14 Feb 2016

Lee Clement M

Abstract excerpt

Fluorescence microscopy is employed to identify Kinesin-1 cargos. Recently, the heavy chain of Kinesin-1 (KIF5B) was shown to transport the nuclear transcription factor c-MYC for proteosomal degradation in the cytoplasm. The method described here involves the study of a motorless KIF5B mutant for fluorescence microscopy. The wild-type and motorless KIF5B proteins are tagged with the fluorescent protein tdTomato....

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Share to Fluorescent Antibody Technique, Indirect
Share to MYC gene

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Microscopy, Fluorescence

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Share to Proto-Oncogene Proteins c-myc

Source

Open in PubMed Open full text

Identifiers

PMID: 26966786
DOI: 10.3791/53632
PMCID: PMC4828159

Genes

MYC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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