Article
Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation.
Nature communications - 9 Mar 2016
Choi Won Hoon, de Poot Stefanie A H, Lee Jung Hoon, Kim Ji Hyeon, Han Dong Hoon, Kim Yun Kyung, Finley Daniel, Lee Min Jae
Abstract excerpt
When in the closed form, the substrate translocation channel of the proteasome core particle (CP) is blocked by the convergent N termini of α-subunits. To probe the role of channel gating in mammalian proteasomes, we deleted the N-terminal tail of α3; the resulting α3ΔN proteasomes are intact but hyperactive in the hydrolysis of fluorogenic peptide substrates and the degradation of polyubiquitinated proteins....
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