Article
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination.
Genetics and molecular research : GMR - 28 Dec 2015
De Azevedo M S P, Santos Rocha C, Pereira V B, De Oliveira Junior A F, De Sousa C S, Azevedo V, LeBlanc J G, Chatel J M, Miyoshi A
Abstract excerpt
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A...
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