Article
Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene.
Cell reports - 26 Jan 2016
Sunagawa Genshiro A, Sumiyama Kenta, Ukai-Tadenuma Maki, Perrin Dimitri, Fujishima Hiroshi, Ukai Hideki, Nishimura Osamu, Shi Shoi, Ohno Rei-Ichiro, Narumi Ryohei, Shimizu Yoshihiro, Tone Daisuke, Ode Koji L, Kuraku Shigehiro, Ueda Hiroki R
Abstract excerpt
The identification of molecular networks at the system level in mammals is accelerated by next-generation mammalian genetics without crossing, which requires both the efficient production of whole-body biallelic knockout (KO) mice in a single generation and high-performance phenotype analyses. Here, we show that the triple targeting of a single gene using the CRISPR/Cas9 system achieves almost perfect KO...
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