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Article

Elimination of truncated recombinant protein expressed in Escherichia coli by removing cryptic translation initiation site.

Protein expression and purification - 1 May 2016

Jennings Matthew J, Barrios Adam F, Tan Song

Abstract excerpt

Undesirable truncated recombinant protein products pose a special expression and purification challenge because such products often share similar chromatographic properties as the desired full length protein. We describe here our observation of both full length and a truncated form of a yeast protein (Gcn5) expressed in Escherichia coli, and the reduction or elimination of the truncated form by mutating a cryptic...

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Identifiers

PMID: 26739786
DOI: 10.1016/j.pep.2015.12.001
PMCID: PMC4803570

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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