Article
Reduced but accurate translation from a mutant AUA initiation codon in the mitochondrial COX2 mRNA of Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 1 Feb 1994
Mulero J J, Fox T D
Abstract excerpt
We have changed the translation initiation codon of the COX2 mRNA of Saccharomyces cerevisiae from AUG to AUA, generating a mutation termed cox2-10. This mutation reduced translation of the COX2 mRNA at least five-fold without affecting the steady-state level of the mRNA, and produced a leaky nonrespiratory growth phenotype. To address the question of whether residual translation of the cox2-10 mRNA was...
Topics
- Base Sequence
- Codon
- Electron Transport Complex IV
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Peptide Chain Initiation, Translational
- Peptide Initiation Factors
- Protein Biosynthesis