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Article

Expression of intron-containing GUS constructs is reduced due to activation of a cryptic 5' splice site.

Molecular genetics and genomics : MGG - 1 May 2001

Ibrahim A F, Watters J A, Clark G P, Thomas C J, Brown J W, Simpson C G

Abstract excerpt

An intron-containing beta-glucuronidase (GUS) gene has been used widely in promoter analyses and as a plant transformation marker. Maximal plant gene expression requires accurate and efficient removal of the intron from the expressed pre-mRNA transcripts by splicing. Detailed analysis of splicing of potato ST-LS1 and pea legumin introns from GUS constructs revealed the activation of a cryptic 5' splice site in...

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PMID: 11405628
DOI: 10.1007/s004380000433

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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