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Article

Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli.

Biotechnology letters - 1 Apr 2016

Ramírez Elisa A, Velázquez Daniela, Lara Alvaro R

Abstract excerpt

OBJECTIVES: To evaluate the combination of a culture medium employing glucoamylase-mediated glucose reléase from a gluco-polysaccharide and an E. coli strain engineered in its glucose transport system for improving plasmid DNA (pDNA) production. RESULTS: The production of pDNA was tested using E. coli DH5α grown in shake-flasks and the recently developed VH33 Δ(recA deoR)-engineered strain, which utilizes glucose...

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Identifiers

PMID: 26696535
DOI: 10.1007/s10529-015-2017-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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