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Article

Enhanced production of plasmid DNA by engineered Escherichia coli strains.

Journal of biotechnology - 30 Apr 2012

Pablos Tania E, Soto René, Mora Eugenio Meza, Le Borgne Sylvie, Ramírez Octavio T, Gosset Guillermo, Lara Alvaro R

Abstract excerpt

Escherichia coli strains VH33 (PTS⁻ GalP⁺ strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of...

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PMID: 21718724
DOI: 10.1016/j.jbiotec.2011.04.015

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PTS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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