Article
Functional cis-expression of phaCAB genes for poly(3-hydroxybutyrate) production by Escherichia coli.
Letters in applied microbiology - 1 May 2011
Horng Y-T, Chien C-C, Wei Y-H, Chen S-Y, Lan J C-W, Sun Y-M, Soo P-C
Abstract excerpt
AIMS: To develop a microbial strain producing poly(3-hydroxybutyrate) [P(3HB)], in the absence of antibiotic supplementation (normally required to stabilize a recombinant plasmid), by constructing a recombinant Escherichia coli strain with phaCAB and vgb integrated into the chromosome. METHODS AND RESULTS: The polyhydroxyalkanoate (PHA) synthesis operon (phaCAB) and the bacterial haemoglobin gene (vgb) were...
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