Article
High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacB by Introducing inc Mutations.
Applied and environmental microbiology - 1 Dec 2014
Trivedi Ram Narayan, Akhtar Parvez, Meade Jonathan, Bartlow Patrick, Ataai Mohammad M, Khan Saleem A, Domach Michael M
Abstract excerpt
For small-copy-number pUC-type plasmids, the inc1 and inc2 mutations, which deregulate replication, were previously found to increase the plasmid copy number 6- to 7-fold. Because plasmids can exert a growth burden, it was not clear if further amplification of copy number would occur due to inc mutations when the starting point for plasmid copy number was orders of magnitude higher. To investigate further the...
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