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Article

Mutating a Highly Conserved Residue in Diverse Cytochrome P450s Facilitates Diastereoselective Olefin Cyclopropanation.

Chembiochem : a European journal of chemical biology - 2 Mar 2016

Gober Joshua G, Rydeen Amy E, Gibson-O'Grady Evan J, Leuthaeuser Janelle B, Fetrow Jacquelyn S, Brustad Eric M

Abstract excerpt

Cytochrome P450s and other heme-containing proteins have recently been shown to have promiscuous activity for the cyclopropanation of olefins using diazoacetate reagents. Despite the progress made thus far, engineering selective catalysts for all possible stereoisomers for the cyclopropanation reaction remains a considerable challenge. Previous investigations of a model P450 (P450BM3 ) revealed that mutation of a...

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PMID: 26690878
DOI: 10.1002/cbic.201500624
PMCID: PMC5241096

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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