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Article

Engineering of p450pyr hydroxylase for the highly regio- and enantioselective subterminal hydroxylation of alkanes.

Angewandte Chemie (International ed. in English) - 17 Mar 2014

Yang Yi, Liu Ji, Li Zhi

Abstract excerpt

Terminal-selective cytochrome P450pyr has been successfully engineered through directed evolution for the subterminal hydroxylation of alkanes with excellent regio- and enantioselectivity. A sensitive colorimetric high-throughput screening (HTS) assay was developed for the measurement of both the regioselectivity and the enantioselectivity of a hydroxylation reaction. By using the HTS assay and iterative...

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PMID: 24554642
DOI: 10.1002/anie.201311091

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