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Article

Improvements to a Markerless Allelic Exchange System for Bacillus anthracis.

PloS one - 1 Jan 2015

Plaut Roger D, Stibitz Scott

Abstract excerpt

A system was previously developed for conducting I-SceI-mediated allelic exchange in Bacillus anthracis. In this system, recombinational loss of a chromosomally-integrated allelic exchange vector is stimulated by creation of a double-stranded break within the vector by the homing endonuclease I-SceI. Although this system is reasonably efficient and represents an improvement in the tools available for allelic...

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Identifiers

PMID: 26624016
DOI: 10.1371/journal.pone.0142758
PMCID: PMC4666636

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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