Article
Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria.
Plasmid - 1 May 2004
Philippe Nadège, Alcaraz Jean-Pierre, Coursange Evelyne, Geiselmann Johannes, Schneider Dominique
Abstract excerpt
Allelic exchange experiments allow investigation of the functions of many unknown genes identified during the sequencing of entire genomes. Isogenic strains differing by only specific mutations can be constructed. Among other tools, suicide plasmids are widely used for this task. They present many advantages because they leave no scars on the chromosome, and therefore allow combining several mutations in the same...
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