Article
Allelic retrieval: a scheme to facilitate the repeated isolation of a specific segment of the Bordetella pertussis chromosome.
Gene - 27 Feb 1998
Stibitz S
Abstract excerpt
A plasmid vector was designed, constructed, and used for the repeated retrieval of the bvgA gene from a number of Bordetella pertussis strains that, due to mutations in this gene, exhibited interesting phenotypes regarding the regulation of virulence genes. The vector was used in a scheme called...
Topics
- Alleles
- Bacterial Proteins
- Bordetella pertussis
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genetic Techniques
- Models, Genetic
- Plasmids
- Restriction Mapping
- Transcription Factors
- Virulence