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Article

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria.

Journal of visualized experiments : JoVE - 2 Feb 2021

Adolfi Adriana, Lynd Amy, Lycett Gareth J, James Anthony A

Abstract excerpt

Functional genomic analysis and related strategies for genetic control of malaria rely on validated and reproducible methods to accurately modify the genome of Anopheles mosquitoes. Amongst these methods, the φC31 system allows precise and stable site-directed integration of transgenes, or the substitution of integrated transgenic cassettes via recombinase-mediated cassette exchange (RMCE). This method relies on...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33616090
DOI: 10.3791/62146

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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