Article
Where does transcription start? 5'-RACE adapted to next-generation sequencing.
Nucleic acids research - 7 Apr 2016
Leenen Fleur A D, Vernocchi Sara, Hunewald Oliver E, Schmitz Stephanie, Molitor Anne M, Muller Claude P, Turner Jonathan D
Abstract excerpt
The variability and complexity of the transcription initiation process was examined by adapting RNA ligase-mediated rapid amplification of 5' cDNA ends (5'-RACE) to Next-Generation Sequencing (NGS). We oligo-labelled 5'-m(7)G-capped mRNA from two genes, the simple mono-exonic Beta-2-Adrenoceptor (ADRB2R)and the complex multi-exonic Glucocorticoid Receptor (GR, NR3C1), and detected a variability in TSS location...
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