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Article

Tuning the Phosphoryl Donor Specificity of Dihydroxyacetone Kinase from ATP to Inorganic Polyphosphate. An Insight from Computational Studies.

International journal of molecular sciences - 24 Nov 2015

Sánchez-Moreno Israel, Bordes Isabel, Castillo Raquel, Ruiz-Pernía José Javier, Moliner Vicent, García-Junceda Eduardo

Abstract excerpt

Dihydroxyacetone (DHA) kinase from Citrobacter freundii provides an easy entry for the preparation of DHA phosphate; a very important C3 building block in nature. To modify the phosphoryl donor specificity of this enzyme from ATP to inorganic polyphosphate (poly-P); a directed evolution program has been initiated. In the first cycle of evolution, the native enzyme was subjected to one round of error-prone PCR...

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Identifiers

PMID: 26610480
DOI: 10.3390/ijms161126073
PMCID: PMC4661931

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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