Article
Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog.
The Journal of biological chemistry - 15 Jan 2016
Tavoulari Sotiria, Margheritis Eleonora, Nagarajan Anu, DeWitt David C, Zhang Yuan-Wei, Rosado Edwin, Ravera Silvia, Rhoades Elizabeth, Forrest Lucy R, Rudnick Gary
Abstract excerpt
In LeuT, a prokaryotic homolog of neurotransmitter transporters, Na(+) stabilizes outward-open conformational states. We examined how each of the two LeuT Na(+) binding sites contributes to Na(+)-dependent closure of the cytoplasmic pathway using biochemical and biophysical assays of conformation. Mutating either of two residues that contribute to the Na2 site completely prevented cytoplasmic closure in response...
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