Article
Production of Human Albumin in Pigs Through CRISPR/Cas9-Mediated Knockin of Human cDNA into Swine Albumin Locus in the Zygotes.
Scientific reports - 12 Nov 2015
Peng Jin, Wang Yong, Jiang Junyi, Zhou Xiaoyang, Song Lei, Wang Lulu, Ding Chen, Qin Jun, Liu Liping, Wang Weihua, Liu Jianqiao, Huang Xingxu, Wei Hong, Zhang Pumin
Abstract excerpt
Precise genome modification in large domesticated animals is desirable under many circumstances. In the past it is only possible through lengthy and burdensome cloning procedures. Here we attempted to achieve that goal through the use of the newest genome-modifying tool CRISPR/Cas9. We set out to knockin human albumin cDNA into pig Alb locus for the production of recombinant human serum albumin (rHSA). HSA is a...
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