Article
New Tricks for Old Proteins: Single Mutations in a Nonenzymatic Protein Give Rise to Various Enzymatic Activities.
Journal of the American Chemical Society - 2 Dec 2015
Moroz Yurii S, Dunston Tiffany T, Makhlynets Olga V, Moroz Olesia V, Wu Yibing, Yoon Jennifer H, Olsen Alissa B, McLaughlin Jaclyn M, Mack Korrie L, Gosavi Pallavi M, van Nuland Nico A J, Korendovych Ivan V
Abstract excerpt
Design of a new catalytic function in proteins, apart from its inherent practical value, is important for fundamental understanding of enzymatic activity. Using a computationally inexpensive, minimalistic approach that focuses on introducing a single highly reactive residue into proteins to achieve catalysis we converted a 74-residue-long C-terminal domain of calmodulin into an efficient esterase. The catalytic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.