Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Selection and Validation of Spacer Sequences for CRISPR-Cas9 Genome Editing and Transcription Regulation in Bacteria.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Grenier Frédéric, Lucier Jean-François, Rodrigue Sébastien

Abstract excerpt

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems have recently been adapted as sequence-programmable tools for various purposes such as genome editing and transcriptional regulation. A critical aspect of the system is the selection and validation of spacer sequences that allow precise targeting of the guide RNA-Cas9 complex. We describe a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Endonucleases

Share to Gene Expression Regulation

Share to Genetic Engineering

Share to Genome, Bacterial

Share to RNA Editing

Share to Transcription, Genetic

Source

Identifiers

PMID: 26404154
DOI: 10.1007/978-1-4939-2877-4_15

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Selection and Validation of Spacer Sequences for CRISPR-Cas9 Genome Editing and Transcription Regulation in Bacteria.PMID 26404154Read ↗

Harnessing CRISPR-Cas systems for bacterial genome editing.Semantic proximity Read ↗

Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids.Semantic proximity Read ↗

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR-Cas system.Semantic proximity Read ↗

Clustered regularly interspaced short palindromic repeats (CRISPRs) for the genotyping of bacterial pathogens.Semantic proximity Read ↗

Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response.Semantic proximity Read ↗

Identification of spacer and protospacer sequence requirements in the <i>Vibrio cholerae</i> Type I-E CRISPR/Cas SystemSemantic proximity Read ↗

CRISPRcompar: a website to compare clustered regularly interspaced short palindromic repeats.Semantic proximity Read ↗

PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systemsSemantic proximity Read ↗

CRISPR interference and priming varies with individual spacer sequences.Semantic proximity Read ↗

RNA-Seq analyses reveal CRISPR RNA processing and regulation patterns.Semantic proximity Read ↗

CRISPR provides acquired resistance against viruses in prokaryotes.Semantic proximity Read ↗

Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.