Article
Clustered regularly interspaced short palindromic repeats (CRISPRs) for the genotyping of bacterial pathogens.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Grissa Ibtissem, Vergnaud Gilles, Pourcel Christine
Abstract excerpt
Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA sequences composed of a succession of repeats (23- to 47-bp long) separated by unique sequences called spacers. Polymorphism can be observed in different strains of a species and may be used for genotyping. We describe protocols and bioinformatics tools that allow the identification of CRISPRs from sequenced genomes, their comparison, and...
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