Article
Detection of hypermutated human papillomavirus type 16 genome by Next-Generation Sequencing.
Virology - 1 Nov 2015
Wakae Kousho, Aoyama Satoru, Wang Zhe, Kitamura Kouichi, Liu Guangyan, Monjurul Ahasan Md, Koura Miki, Imayasu Mieko, Sakamoto Naoya, Nakamura Mitsuhiro, Kyo Satoru, Kondo Satoru, Fujiwara Hiroshi, Yoshizaki Tomokazu, Kukimoto Iwao, Yamaguchi Katsushi, Shigenobu Shuji, Nishiyama Tomoaki, Muramatsu Masamichi
Abstract excerpt
Human papillomavirus type 16 (HPV16) is a major cause of cervical cancer. We previously demonstrated that C-to-T and G-to-A hypermutations accumulated in the HPV16 genome by APOBEC3 expression in vitro. To investigate in vivo characteristics of hypermutation, differential DNA denaturation-PCR (3D-PCR) was performed using three clinical specimens obtained from HPV16-positive cervical dysplasia, and detected...
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