Article
Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers.
Proceedings of the National Academy of Sciences of the United States of America - 22 Sept 2015
Budeus Bettina, Schweigle de Reynoso Stefanie, Przekopowitz Martina, Hoffmann Daniel, Seifert Marc, Küppers Ralf
Abstract excerpt
Our knowledge about the clonal composition and intraclonal diversity of the human memory B-cell compartment and the relationship between memory B-cell subsets is still limited, although these are central issues for our understanding of adaptive immunity. We performed a deep sequencing analysis of rearranged immunoglobulin (Ig) heavy chain genes from biological replicates, covering more than 100,000 memory B...
Topics
- Adaptive Immunity
- Adult
- B-Lymphocytes
- Cell Separation
- Computational Biology
- DNA Mutational Analysis
- Germinal Center
- Humans
- Immunoglobulin Class Switching
- Immunoglobulin D