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Article

Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.

Proceedings of the National Academy of Sciences of the United States of America - 8 Sept 2015

Bruck Irina, Kaplan Daniel L

Abstract excerpt

Dbf4-dependent kinase (DDK) phosphorylates minichromosome maintenance 2 (Mcm2) during S phase in yeast, and Sld3 recruits cell division cycle 45 (Cdc45) to minichromosome maintenance 2-7 (Mcm2-7). We show here DDK-phosphoryled Mcm2 preferentially interacts with Cdc45 in vivo, and that Sld3 stimulates DDK phosphorylation of Mcm2 by 11-fold. We identified a mutation of the replication initiation factor Sld3,...

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Identifiers

PMID: 26305950
DOI: 10.1073/pnas.1509608112
PMCID: PMC4568703

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.PMID 26305950Read ↗

Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7.Semantic proximity Read ↗

SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.Semantic proximity Read ↗

Concerted activities of Mcm4, Sld3, and Dbf4 in control of origin activation and DNA replication fork progression.Semantic proximity Read ↗

Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression.Semantic proximity Read ↗

Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.Semantic proximity Read ↗

Dbf4 and Cdc7 proteins promote DNA replication through interactions with distinct Mcm2-7 protein subunits.Semantic proximity Read ↗

Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.Semantic proximity Read ↗

A cell cycle-regulated Slx4-Dpb11 complex promotes the resolution of DNA repair intermediates linked to stalled replication.Semantic proximity Read ↗

Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction.Semantic proximity Read ↗

Histone H3 Thr 45 phosphorylation is a replication-associated post-translational modification in S. cerevisiae.Semantic proximity Read ↗

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.Semantic proximity Read ↗

CDK phosphorylation of SLD-2 is required for replication initiation and germline development in C. elegans.Semantic proximity Read ↗

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