Article
Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens.
Journal of clinical microbiology - 1 Nov 2015
Peterson S W, Martin I, Demczuk W, Bharat A, Hoang L, Wylie J, Allen V, Lefebvre B, Tyrrell G, Horsman G, Haldane D, Garceau R, Wong T, Mulvey M R
Abstract excerpt
We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.
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