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Article

Mismatch Amplification Mutation Assay-Based Real-Time PCR for Rapid Detection of Neisseria gonorrhoeae and Antimicrobial Resistance Determinants in Clinical Specimens.

Journal of clinical microbiology - 1 Sept 2018

Donà Valentina, Smid Joost H, Kasraian Sara, Egli-Gany Dianne, Dost Ferah, Imeri Fatime, Unemo Magnus, Low Nicola, Endimiani Andrea

Abstract excerpt

Molecular methods are often used for Neisseria gonorrhoeae detection, but complete definition of antimicrobial resistance (AMR) patterns still requires phenotypic tests. We developed an assay that both identifies N. gonorrhoeae and detects AMR determinants in clinical specimens. We designed a mismatch amplification mutation assay (MAMA)-based SYBR green real-time PCR targeting one N. gonorrhoeae-specific region...

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Bacterial Outer Membrane Proteins
False Positive Reactions
Gonorrhea
Molecular Diagnostic Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Neisseria gonorrhoeae

Real-Time Polymerase Chain Reaction

Specimen Handling

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Open in PubMed Open full text

Identifiers

PMID: 29950339
DOI: 10.1128/JCM.00365-18
PMCID: PMC6113480

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