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Article

Multiplex Real-Time PCR Assay for Simultaneous Identification of Neisseria gonorrhoeae and Its Ciprofloxacin Susceptibility Status.

Journal of clinical microbiology - 1 Nov 2017

Perera Sumudu R, Khan Nurul H, Martin Irene, Taheri Ali, Parti Rajinder P, Levett Paul N, Horsman Greg B, Kusalik Anthony, Dillon Jo-Anne R

Abstract excerpt

A real-time PCR (RT-PCR) assay was designed for the simultaneous identification of Neisseria gonorrhoeae and its ciprofloxacin susceptibility status. A SYBR green-based multiplex RT-PCR format was used; it comprised two different forward primers and a common reverse primer to detect single nucleotide polymorphisms (SNPs) in gyrA of N. gonorrhoeae The primer pairs were evaluated for their sensitivity and...

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Identifiers

PMID: 28814585
DOI: 10.1128/JCM.00855-17
PMCID: PMC5654903

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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