Article
Single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly.
Proceedings of the National Academy of Sciences of the United States of America - 25 Aug 2015
Arai Taiga, Ishiguro Kensuke, Kimura Satoshi, Sakaguchi Yuriko, Suzuki Takeo, Suzuki Tsutomu
Abstract excerpt
Ribosome biogenesis requires multiple assembly factors. In Escherichia coli, deletion of RlmE, the methyltransferase responsible for the 2'-O-methyluridine modification at position 2552 (Um2552) in helix 92 of the 23S rRNA, results in slow growth and accumulation of the 45S particle. We demonstrate that the 45S particle that accumulates in ΔrlmE is a genuine precursor that can be assembled into the 50S subunit....
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