Article
Translational defects of Escherichia coli mutants deficient in the Um(2552) 23S ribosomal RNA methyltransferase RrmJ/FTSJ.
Biochemical and biophysical research communications - 19 May 2000
Caldas T, Binet E, Bouloc P, Richarme G
Abstract excerpt
We recently identified RrmJ (alias FtsJ), the first encoded protein of the rrmJ-hflB heat shock operon, as an Um(2552) methyltransferase of the 23S rRNA. We now report that the rrmJ-deficient strain exhibits growth and translational defects compared to the wild-type strain. Growth rates of the rrmJ mutant are decreased at both low and high temperatures. Protein synthesis activity is reduced up to 65% when S(30)...
Topics
- Anti-Bacterial Agents
- Bacterial Proteins
- Cell Cycle Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Kinetics
- Lac Operon
- Methyltransferases
- Mutation
- Polyribosomes
- Protein Biosynthesis
- RNA, Ribosomal, 23S
- Ribosomes
- Temperature