Article
A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes.
Scientific reports - 9 Aug 2018
Sharma Sunny, Hartmann Johannes David, Watzinger Peter, Klepper Arvid, Peifer Christian, Kötter Peter, Lafontaine Denis L J, Entian Karl-Dieter
Abstract excerpt
The entire chemical modification repertoire of yeast ribosomal RNAs and the enzymes responsible for it have recently been identified. Nonetheless, in most cases the precise roles played by these chemical modifications in ribosome structure, function and regulation remain totally unclear. Previously, we demonstrated that yeast Rrp8 methylates m1A645 of 25S rRNA in yeast. Here, using mung bean nuclease protection...
Topics
- Adenosine
- Base Sequence
- Electrophoresis, Gel, Two-Dimensional
- HCT116 Cells
- Humans
- Methylation
- Methyltransferases
- Mutation
- Nuclear Proteins
- Nucleic Acid Conformation
- Protein Domains
- Protein O-Methyltransferase
- Proteomics