Article
Approaches to Characterize and Quantify Oligomerization of GPCRs.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Marsango Sara, Varela María José, Milligan Graeme
Abstract excerpt
Fluorescence resonance energy transfer (FRET) is an approach widely used to detect protein-protein interactions in live cells. This approach is based on the sensitization of an "acceptor" molecule by the energy transfer from a "donor" when there is an overlap between the emission spectrum of the "donor" and the excitation spectrum of the "acceptor" and close proximity between the two fluorophore species (in the...
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