Article
Oligomerization of dopamine transporters visualized in living cells by fluorescence resonance energy transfer microscopy.
The Journal of biological chemistry - 25 Jul 2003
Sorkina Tatiana, Doolen Suzanne, Galperin Emilia, Zahniser Nancy R, Sorkin Alexander
Abstract excerpt
To examine the oligomeric state and trafficking of the dopamine transporter (DAT) in different compartments of living cells, human DAT was fused to yellow (YFP) or cyan fluorescent protein (CFP). YFP-DAT and CFP-DAT were transiently and stably expressed in porcine aortic endothelial (PAE) cells, human embryonic kidney (HEK) 293 cells, and an immortalized dopaminergic cell line 1RB3AN27. Fluorescence microscopic...
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